Approximately 600 different compounds were profiled in up to 7 different rat tissues by obtaining tissue samples from test compound-treated and vehicle control-treated rats in biological triplicates for gene expression analysis after 0.25, 1, 3, and 5 days of exposure with daily dosing. In a few studies (1.8%), 7 days of exposure was substituted for 5 days of exposure. Samples were hybridized to whole genome RG230_2.0 GeneChip arrays (Affymetrix, CA). DrugMatrix is a comprehensive rat toxicogenomics database and analysis tool developed to facilitate the integration of toxicogenomics into hazard assessment. Using the whole genome and a diverse set of compounds allows a comprehensive view of most pharmacological and toxicological questions and is applicable to other situations such as disease and development. Male Sprague–Dawley (Crl:CD (SD)|GS BR) rats(aged 6–8 weeks) were purchased from Charles River Laboratories (Wilmington, MA). They were housed in plastic cages for 1 week for acclimation to the laboratory environment of a ventilated room (temperature, 22 +- 3 C; humidity 30–70%; 12-h light:12-h dark cycle per day, 6:00 a.m.- 6:00 p.m.) until use. Certified Rodent Diet #5002 (PMI Feeds Inc.) and chlorinated tap water was available ad libitum.
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