Organism | Mus musculus | |
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Tissue | liver |
Experimental type | In-vivo |
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Sex | Male |
Generation | F0 |
Developmental stage | Adulthood |
Expression data from liver of mice treated with different coumarins (mammea and soulatrolide) from Calophyllum brasiliense. 12 Total samples were analyzed. Liver from 4 animals per treatment groups were subjected to mRNA expression analysis. We generated the statistical computing and metrics in the arrayQualityMetrics data were normalized with RMA method and differential expressed genes obtained with Ingenuity Software. Genes with an FDR≤10% and a fold-change ≥2 were selected.
Liver of animals was immediately obtained after sacrifice and samples immediately homogenized with PureLink RNA Mini Kit. Integrity of RNA was determinated by using a Bioanalyzer