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TSA8493 - Effect of Chlorpyrifos at 3 mg/kg 21 days Public Interventional
Summary
| Organism |
|
Mus musculus |
|---|---|---|
| Tissue | layer of hippocampus |
Characteristics
| Experimental type | In-vivo |
|---|---|
| Sex | Male |
| Generation | F0 |
| Developmental stage | Adulthood |
Experimental design
Adult male Long Evans rats were repeatedly exposed to subclinical doses of chlorpyrifos or vehicle control (peanut oil) for 21 consecutive days (study days 1-21). Two CPF formulations (3 or 10 mg/kg b.w./day) or an equal volume of the vehicle were administered daily by subcutaneous injection, approximately 24 h apart. On the study day 21, animals were euthanized and hippocampus tissues were collected for biochemical and transcriptomic analyses. The global RNA profiles of all test animals were obtained using the Affymetrix GeneChip microarrays. The Illumina sequencing technologies were employed to analyzed comprehensive profiles of mRNAs and small RNAs from the rats exposed to 10 mg/kg/day CPF or the unexposed control rats.
Additional information
Total RNA including small non-coding RNA was isolated from the CA1 region of rat hippocampus using miRNeasy kit (Qiagen, Valencia, CA) according to the manufacturer’s protocol.