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TSA9750 - CMap - carcinogenome project: Toxicogenomic signatures in the human cell line MCF10A.WT.HP after exposure to benzo[k]fluoranthene Public
Summary
| Organism |
|
Homo sapiens |
|---|---|---|
| Tissue | breast | |
| Cell |  | Epithelial cell |
| Cell line | | Breast fibrocystic disease |
Characteristics
| Experimental type | In-vitro |
|---|---|
| Sex | Na |
| Generation | F0 |
| Developmental stage | Na |
Experimental design
In the carcinogenome project, cells are exposed to each individual chemical for 24 hours and their gene expression is profiled on the L1000 platform. Each chemical is assayed at 6 doses (2 fold dilutions starting from the highest concentration of 40uM or 20uM) with triplicate profiles generated for each dose. For each chemical and dose profile, the gene expression of 1000 landmark genes is calculated as a moderated z-score (weighted collapsed z-score of the 3 replicate perturbational profiles with respect to the entire plate). (https://carcinogenome.org/HEPG2/)
Additional information
No additional information provided